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  • Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

    Cheap and easy: That kind of technology is the best!


    Apr. 17, 2013 — Hydrogen sulfide, the pungent stuff often referred to as sewer gas, is a deadly substance implicated in several mass extinctions, including one at the end of the Permian period 251 million years ago that wiped out more than three-quarters of all species on Earth


    But in low doses, hydrogen sulfide could greatly enhance plant growth, leading to a sharp increase in global food supplies and plentiful stock for biofuel production, new University of Washington research shows.

    "We found some very interesting things, including that at the very lowest levels plant health improves. But that's not what we were looking for," said Frederick Dooley, a UW doctoral student in biology who led the research.

    Dooley started off to examine the toxic effects of hydrogen sulfide on plants but mistakenly used only one-tenth the amount of the toxin he had intended. The results were so unbelievable that he repeated the experiment. Still unconvinced, he repeated it again -- and again, and again. In fact, the results have been replicated so often that they are now "a near certainty," he said.

    "Everything else that's ever been done on plants was looking at hydrogen sulfide in high concentrations," he said.

    The research is published online April 17 in PLOS ONE, a Public Library of Science journal.At high concentrations -- levels of 30 to 100 parts per million in water -- hydrogen sulfide can be lethal to humans. At one part per million it emits a telltale rotten-egg smell. Dooley used a concentration of 1 part per billion or less to water seeds of peas, beans and wheat on a weekly basis. Treating the seeds less often reduced the effect, and watering more often typically killed them.With wheat, all the seeds germinated in one to two days instead of four or five, and with peas and beans the typical 40 percent rate of germination rose to 60 to 70 percent.

    "They germinate faster and they produce roots and leaves faster. Basically what we've done is accelerate the entire plant process," he said.Crop yields nearly doubled, said Peter Ward, Dooley's doctoral adviser, a UW professor of biology and of Earth and space sciences and an authority on Earth's mass extinctions.

    Hydrogen sulfide, probably produced when sulfates in the oceans were decomposed by sulfur bacteria, is believed to have played a significant role in several extinction events, in particular the "Great Dying" at the end of the Permian period. Ward suggests that the rapid plant growth could be the result of genetic signaling passed down in the wake of mass extinctions.At high concentrations, hydrogen sulfide killed small plants very easily while larger plants had a better chance at survival, he said, so it is likely that plants carry a defense mechanism that spurs their growth when they sense hydrogen sulfide.

    "Mass extinctions kill a lot of stuff, but here's a legacy that promotes life," Ward said.

    Dooley recently has applied hydrogen sulfide treatment to corn, carrots and soybeans with results that appear to be similar to earlier tests. But it is likely to be some time before he, and the general public, are comfortable with the level of testing to make sure there are no unforeseen consequences of treating food crops with hydrogen sulfide.

    The most significant near-term promise, he believes, is in growing algae and other stock for biofuels. Plant lipids are the key to biofuel production, and preliminary tests show that the composition of lipids in hydrogen sulfide-treated plants is the same as in untreated plants, he said.

    When plants grow to larger-than-normal size, they typically do not produce more cells but rather elongate their existing cells, Dooley said. However, in the treatment with hydrogen sulfide, he found that the cells actually got smaller and there were vastly more of them. That means the plants contain significantly more biomass for fuel production, he said.
    "If you look at a slide of the cells under a microscope, anyone can understand it. It is that big of a difference," he said.

    Ward and Suven Nair, a UW biology undergraduate, are coauthors of the PLOS ONE paper. The work was funded by the UW Astrobiology Program.


    http://www.sciencedaily.com/releases...0417185531.htm

  • #2
    Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

    Many of the deep structural natural gas reservoirs along the North American Rocky Mountains contain abundant quantities of hydrogen sulfide (H2S). It's a source of extremely pure elemental sulphur (bright yellow) when the gas is processed. The sulphur is then used to make sulfuric acid which is reacted with phosphor rock to make phosphate fertilizer (the "P" in N-P-K).

    The article doesn't mention this, but I wonder if all that is happening here is that the slightly acid result of dissolving H2S in water is offsetting an alkaline growing media/soil.

    Comment


    • #3
      Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

      Originally posted by GRG55 View Post
      Many of the deep structural natural gas reservoirs along the North American Rocky Mountains contain abundant quantities of hydrogen sulfide (H2S). It's a source of extremely pure elemental sulphur (bright yellow) when the gas is processed. The sulphur is then used to make sulfuric acid which is reacted with phosphor rock to make phosphate fertilizer (the "P" in N-P-K).

      The article doesn't mention this, but I wonder if all that is happening here is that the slightly acid result of dissolving H2S in water is offsetting an alkaline growing media/soil.
      I was also wondering this. I know that we tend to obsess about the energy-intensive Nitrogen-rich fertilizers made using the Haber-Bosch process. But plants, depending on their environment, may also be limited by the phosphate, potassium (potash), or among others, sulfur content of their soil.

      A detailed table of nutrient extraction is found here.

      If the tests were conducted in a sulfur-poor environment (relative to the needs of the plant species tested) that could account for similar effects.

      And as far as algae phospholipids densities go, I would expect phosphates to have a much bigger effect than sulphur compounds. That doesn't mean hydrogen sulfide can't help as well, but it does seem that one would want to test the concept on the correct species before making claims about biofuel algae.

      Comment


      • #4
        Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

        Originally posted by astonas View Post
        I was also wondering this. I know that we tend to obsess about the energy-intensive Nitrogen-rich fertilizers made using the Haber-Bosch process. But plants, depending on their environment, may also be limited by the phosphate, potassium (potash), or among others, sulfur content of their soil.

        A detailed table of nutrient extraction is found here.

        If the tests were conducted in a sulfur-poor environment (relative to the needs of the plant species tested) that could account for similar effects.

        And as far as algae phospholipids densities go, I would expect phosphates to have a much bigger effect than sulphur compounds. That doesn't mean hydrogen sulfide can't help as well, but it does seem that one would want to test the concept on the correct species before making claims about biofuel algae.
        'we tend to obsess about the energy-intensive Nitrogen-rich fertilizers made using the Haber-Bosch process'

        bwaha ha ha! only on itulip!

        Comment


        • #5
          Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

          Originally posted by metalman View Post
          'we tend to obsess about the energy-intensive Nitrogen-rich fertilizers made using the Haber-Bosch process'

          bwaha ha ha! only on itulip!
          heh, I suppose the language was a bit strong... "Emphasize" would be more accurate than "obsess." Sorry for implying we're not balanced in that regard, it does not accurately represent my view.

          But I think the point that fertilizers that don't work principally toward fixing nitrogen can still be growth-limiting stands. It just doesn't tie back as directly to PCO, so it understandably gets less play here.

          Comment


          • #6
            Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

            Here is the original manuscript.
            http://www.plosone.org/article/info%...l.pone.0062048

            Its an interesting paper however I don't think its going to be a be all to end all. If we want to improve biomass, plant productivity, etc. I think its going to be done through engineering better Rubisco or conversion of C3 to C4 or modifying the photosynthetic antenna complex, or various other routes of plant bioengineering.

            I am also not sure that I buy the authors assertion that its an evolutionary response to prehistoric catastrophic levels of atmospheric changes during the mass extinctions, nonetheless its still pretty interesting.
            Last edited by Guinnesstime; April 19, 2013, 04:22 AM. Reason: Habitutal Bad Spelling and Grammar

            Comment


            • #7
              Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

              Originally posted by Guinnesstime View Post
              Here is the original manuscript.
              http://www.plosone.org/article/info%...l.pone.0062048

              Its an interesting paper however I don't think its going to be a be all to end all. If we want to improve biomass, plant productivity, etc. I think its going to been done through engineering better Rubisco or conversion of C3 to C4 or modifying the photosynthetic antenna complex, or various other routes of plant bioengineering.

              I am also not sure that I buy the authors assertion that its an evolutionary response to prehistoric catastrophic levels of atmospheric changes during the mass extinctions, nonetheless its still pretty interesting.
              My understanding was that the photosynthetic antenna complex was already sufficiently efficient to not be rate-limiting. Is this incorrect?

              Thanks for linking the original, I'm slightly more inclined now to agree that a real signaling effect may be present, though the mechanism that would employ is still unclear to me. Thus, the jury is still out (for me) on both overall significance, and applicability to biofuel algae.

              I'm not really in a position to comment on evolutionary origins, since I know very little about early atmospheric compositions.
              Last edited by astonas; April 19, 2013, 01:17 AM.

              Comment


              • #8
                Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

                Originally posted by metalman View Post
                'we tend to obsess about the energy-intensive Nitrogen-rich fertilizers made using the Haber-Bosch process'

                bwaha ha ha! only on itulip!
                MM: If you are not obsessing about this important topic your life is incomplete and an utter sham. Get with the program, eh.

                P.S. The government will be sure to let you know when it's time to obsess about something else that's important...

                Comment


                • #9
                  Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

                  Originally posted by astonas View Post
                  My understanding was that the photosynthetic antenna complex was already sufficiently efficient to not be rate-limiting. Is this incorrect?

                  Thanks for linking the original, I'm slightly more inclined now to agree that a real signaling effect may be present, though the mechanism that would employ is still unclear to me. Thus, the jury is still out (for me) on both overall significance, and applicability to biofuel algae.

                  I'm not really in a position to comment on evolutionary origins, since I know very little about early atmospheric compositions.
                  If you can shorten the antenna complex then you could improve light penetration and mitigate light saturation. Some of this work has been done on micro algae, but I don't know if anyone has done this for higher plant life.
                  Last edited by Guinnesstime; April 19, 2013, 04:23 AM. Reason: Poor Grammar as Usual

                  Comment


                  • #10
                    Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

                    Originally posted by Guinnesstime View Post
                    If you can shorten the antenna complex then you could improve light penetration and mitigate light saturation. Some of this work has been done on micro algae, but I don't know if anyone has done this for higher plant life.
                    Makes perfect sense! Thanks for the insight! It hadn't occured to me that making the collection stage less efficient might be so advantageous, but I can see how it would increase the overall productive absorption. You could get a much thicker active algae mat, and simultaneously lower exciton conversion losses, as long as the process isn't kinetically-limited in the initial steps of conversion. Is this the case for C3, C4, and CAM photosynthetic pathways?

                    Making it work for higher plant life would be a lot more complicated, since you'd also need to alter signaling to increase the number of cells in the photosynthetic layer, and that in turn would also negatively affect respiration, and dissolved gas distribution in general. I'm not entirely sure the advantages would be the same as for algae, and the complexity would be far greater. (Unless the signal really is as simple as hydrogen sulfide, which to my mind requires further justification.)


                    Algae does seem to be an ideal candidate, and not just for antennae optimization!

                    Comment


                    • #11
                      Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

                      This seems easy to test.

                      Apply rotten eggs to one half of a test field.

                      Comment


                      • #12
                        Re: Hydrogen Sulfide Greatly Enhances Plant Growth Boost Food, Biofuel Production

                        Originally posted by astonas View Post
                        Makes perfect sense! Thanks for the insight! It hadn't occured to me that making the collection stage less efficient might be so advantageous, but I can see how it would increase the overall productive absorption. You could get a much thicker active algae mat, and simultaneously lower exciton conversion losses, as long as the process isn't kinetically-limited in the initial steps of conversion. Is this the case for C3, C4, and CAM photosynthetic pathways?

                        Making it work for higher plant life would be a lot more complicated, since you'd also need to alter signaling to increase the number of cells in the photosynthetic layer, and that in turn would also negatively affect respiration, and dissolved gas distribution in general. I'm not entirely sure the advantages would be the same as for algae, and the complexity would be far greater. (Unless the signal really is as simple as hydrogen sulfide, which to my mind requires further justification.)


                        Algae does seem to be an ideal candidate, and not just for antennae optimization!
                        You could reduce antenna size for plants of all pathways to achieve the same goal. However, each pathway handles the conversion process slightly differently with Rubisco being a rate limiting step for all. The whole photosynthetic process if very complex and I believe it will take a multifaceted engineering approach to true make gains, at least for higher plant life. I would agree that algae are an ideal candidate, and I believe there has been more success in photobiology using algae, but I could be wrong.
                        Last edited by Guinnesstime; April 19, 2013, 12:08 PM.

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